International Journal of Current Research in Chemistry and Pharmaceutical Sciences

The present investigation was carried out to study the induced mutagenesis of EMS (Ethyl methane sulphonate) on the seed germination, seedling survival and LD50 value of Catharanthus roseus. The seeds of Vinca rosea were treated with different concentration of EMS viz., 5, 10, 15, 20, 25, 30, 35, 40, 45 and 50 mM. The untreated seeds were taken as control. The EMS treated and control seeds were kept in petriplates and allowed for germination, seed germination and seedling survival percentage were calculated. The germination percentage was calculated on 15 day after sowing and seedling survival proportion at 30 day. The 50 per cent reduction of seed germination was observed as LD50 (lethal dosage) value was calculated at 15 day after sowing. The 50 per cent reduction of seed germination in 25KR of EMS treatment. The seedling survival also decreased with increase in the concentration of EMS.